This figure shows the zwitterionic forms of the chelex resin as a function of ph. Diagnosis of ak is based on the culture of corneal scrapings or detection of the organism in pathology specimens 1. Fight larger fires with water spray or alcohol resistant foam. Identification of the substances mixture and of the company undertaking 1. May be absorbed into the bloodstream with symptoms similar to ingestion. Material safety data sheets low viscosity a resin page 3 of 6 xii. Butyl alcohols have produced few cases of poisoning in industry because of their low volatility. Because this is the first step towards pcr and amplifying your template dna. The pcr product yield was a 1,075 bp from mitochondrial.
Chelex chelating resin is efficiently regenerated in dilute acid and operates in basic, neutral, and weakly acidic solutions of ph 4 or higher. This product and all others supplied by madison chemical co. Pdf a dna extraction method using chelex 100 is widely used for bacteria. Nov 28, 2017 in most cases the resin matrix is composed of polystyrene, though a variety of substances are used for functional groups, including thiol, triethylammonium, and aminophosphonic, among many others. Dna was extracted by using chelex resin according to iovieno et al. The information offered here is for the product as shipped. Page 16 safety data sheet according to 19072006ec, article 31.
Extraction of dna by using anionexchange resin chelex. We morphologically identified 72 anopheles gambiae sl. Higher concentrations can produce central nervous system depressi on, narcosis, and unconsciousness. Extraction of hgii from aqueous solutions by chelating resin chelex 100 section bresearch paper eur. Pdf rapid and simple method for dna extraction from plant and. Hazard description not applicable information pertaining to particular dangers for man and environment not applicable. Then add 5 grams of chelex and fill to 50ml mark with water. Safety data sheet chelex 100 page 2 of 3 no special spill cleanup considerations. Epoxy resin 25068386 ne ne 6080 no alkyl glycidyl ether 120547526 ne ne 1l0 alkyl phenol 84852153 ne ne 110 no benzyl alcohol 100516 ne ne 110 no substances listed are present in concentration of 1% or greater, or 0. Choose biotechnology grade chelex 100 resin, a weak cation exchange media, for chelation, dna extraction for pcr, and other cation exchange protocols. Use this sodiumform resin for gravity or lowpressure chromatography.
May 4, 2000 msds no 70032000 consult an expert on disposal of recovered material and ensure conformity to local disposal regulations. Material safety data sheet page 1 of 1 thank you for your interest in, and use of, this product. It is noteworthy for its ability to bind transition metal ions it is a styrenedivinylbenzene copolymer containing iminodiacetic acid groups. Chelex 100 chelating resin, biotechnology grade, 100200 mesh. Canadian ingredient disclosure list section 16 additional information msds creation date. The information in this document is based on the present state of our knowledge and is applicable to the. May cause coughing, dizziness, dullness, and headache. Improved method for dna extraction and purification from. The manufacturer does not recommend using any kresin sbc grade in medical.
To fully understand how ix resins work, it is important to first understand the principles of the ion exchange. Chelating resin crosslinked polystyrene beads, iminodiacetic acid. Material safety data sheet exxonmobil chemical company a division of exxon mobil corporation page. Safety data sheet ez 10a epoxy resin page 2 of 8 h411 toxic to aquatic life with long lasting effects. Identification of the substancemixture and of the companyundertaking 1. Inhalation of vapors irritates the respiratory tract. At very low ph, the resin acts as an anion exchanger.
Dowex monosphere 650c h ion exchange resin page 7 of 7 revision date 07172012 the information provided in this safety data sheet is correct to the best of our knowledge, information. I want to know their experiences about using this resin and the advantages or disadvantages of this method. Material safety data sheet according to regulation ec no. Here we present a simple chelex based technique for extracting malaria parasite and vector dna from field collected mosquito specimens. The most effective way to do this is to take a 50 ml sterile falcon tube, place in on a scale inside a small beaker and zero the scale. Product identifier product name polyester resin product number por001, por002 internal identification c 1. Sigmaaldrich material safety data sheet date printed. The extraction of dna by the chelex 100 matrix apd complex and. Handling and storage handling general advice product should not be mixed with air above atmospheric pressure for leak testing or any other purpose. Disposal considerations waste from residues this material is not a hazardous waste by rcra criteria 40 cfr 261. Chelex 100 resin is a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. Extraction of acanthamoeba dna by use of chelex resin acanthamoeba keratitis ak is a rare corneal infection caused by the freeliving amoeba acanthamoeba 1.
Certificate of analysis coa product information sheet pdf specification. Chelex resin dna extraction appears to be a sensitive and reliable method for acanthamoeba dna extraction from culture or corneal scrapings and may represent a fast and inexpensive alternative to commercial kits for rapid molecular diagnosis of ak or genotyping of acanthamoeba strains. However, we make no warranty of merchantability or any other warranty, express or implied, w ith respect to such information, and we assume no liability resulting from its use. Page 16 safety data sheet according to 19072006ec, article 31 printing date 22. Bt chelex 100 resin sodium form msds download health.
Page 3 fl 2000 resin msds fl 2000 resin all grades continued rev. Difficult breathing, coughing, headache, dizziness, and drowsiness may occur. Chelex 100 and chelex 20 chelating ion exchange resin instruction manual. A modification of the limited bath technicne was used, in which resin was added to a solution containing the trace element. The resin may be rehydrated and used, as long as it remains white. However, the water wash from the chelex resin is very basic. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Clear photoreactive resin for formlabs 3d printers formlabs. Chelex 100 as a medium for simple extraction of dna for pcr. Chelex 100 sodium form 50100 mesh dry sigmaaldrich. This resin may be handled, shipped and stored at elevated temperatures in bulk. Page 3 lpa 2800 resin msds lpa 2800 resin all grades continued rev. Prepare a 510% by weight slurry of chelex100 resin biorad part 143 3832,100200 mesh chelex, sodium form and uv sterilized hplc water. Extraction of acanthamoeba dna by use of chelex resin.
Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Chelex is designed to help the body rid itself of damaging oxidative elements. Safety data sheet according to 19072006ec, article 31 iso. Culture positivity and histology may require a long time 3 to 7. Chelex 100 was evaluated as an ionexchange resin for the extraction of trace elcneits from seawater. The carboxylic acid groups of chelex 100 resin classify it as a weak cation exchange resin, but it differs from other exchangers in this class by featuring uniquely high selectivities for.
Fsp1934a7 page 3 of 8 printed 7may10 089005 compiled 20100507 unsuitable extinguishing media do not use water jet. Recover the spilled material and place in suitable containers. Chelex dna extraction method specialized topicsspring 2008 supplies. A suspension of chelex 100 sodium form in water typically has a ph of 1011. The most effective way to do this is to take a 50 ml sterile falcon tube. Ii and cd ii extraction by chelex 100, by varying diverse parameters as the initial mixture ion concentration, initial ph of aqueous solution, ion strength and the amounts of resin. Procedures utilizing chelex100 chelating resin have been developed for extracting dna from forensictype samples for use with the pcr. Dna extraction methods open university of sri lanka. Chelex 100 as a medium for simple extraction of dna for pcrbased typing from forensic material. Hilti hitre 500sd container size 330 ml, 500 ml, 1400 ml. Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents. Chelex this is a fast, cheap, and effective method of dna extraction. Chelex 100 resin trisedta 1x scale and weigh boat small bottle for storage 1. Chelex 100 as a medium for simple extraction of dna for.
Prepare a 510% by weight slurry of chelex 100 resin biorad part 1433832,100200 mesh chelex, sodium form and uv sterilized hplc water. Dna extraction experiences using chelex 100 from insects especially coleoptea. Chelex extraction chelex 100, molecular biology grade resin from biorad is a highly pure, nuclease and ligase inhibitorfree chelating resin, certified not to interfere with downstream pcr. Chelex 100 safety data sheet msds catalog service llc. Removal of cd ii and hgii by chelating resin chelex100. If the color of the reswollen resin is yellow, do not use it. Chelex 100 chelating resin, biotechnology grade, 100200. Page 16 material safety data sheet idaho state university. Ironiii chelex resin complex as a prototypical heterogeneous catalyst for phosphodiester hydrolysis vasiliki lykourinoutibbs, altan ercan, lijune ming department of chemistry and institute for biomolecular science, university of south florida, 4202 fowler. Material safety data sheet kresin copolymers february 15, 2002. Further evaluations of chelex dna extraction in a clinical. Chelex 100 sodium form 200400 mesh particle size sigmaaldrich.
Sigma c7901 page 6 of 6 the above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Dna extraction by chelex 100 resin apd complex and sdschloroform. Stable when stored in original container designed for use with light sensitive materials under 35 c 95 f in dark, cool place. Dna extraction experiences using chelex 100 from insects. With bleach, sterilize a dry reagent spatula, and a small magnetic stir bar. Use dry nitrogen to transfer or leak test equipment pressurized with product. During the extraction process, the alkalinity of the solution and the act. Regulations and the msds contains all of the information required by those regulations. Special hazard products moderate to severe explosion hazard in confined spaces. Pdf extraction of acanthamoeba dna by use of chelex.
It features specialized ingredients, such as edta, himalayan shilajit extract 50% fulvic acid, chlorella, coriander, and antioxidantstimulating nutrients. Sigmaaldrich offers a number of chelex 100 sodium form products. Materials and methods chelex 100 biorad laboratories, ca, usa is a chelating resin which uses ion exchange to bind transition metal ions. How to perform dna extraction from dried blood spots using. Safety data sheet northwest missouri state university. A comparison of methods for forensic dna extraction.
It is noteworthy for its ability to bind transition metal ions it is a styrenedivinylbenzene copolymer containing iminodiacetic acid groups a concentrated solution of metals is obtained by eluting the resin with a small volume of 2 m nitric acid, which protonates the iminodiacetate groups. The resin is composed of styrene divinylbenzene copolymers containing paired iminodiacetate ions, which act as chelators for polyvalent metal ions. A onestep microbial dna extraction method using chelex. Our msds database is an easy to use, do it yourself tool, and best of all. Relevant identified uses of the substance or mixture and uses advised against identified uses polyester resin. Chelex 100 is a chelating material from biorad used to purify other compounds via ion exchange. This material is classified as an article and not subject to tsca.
How to perform dna extraction from dried blood spots using chelex resin published may 16, 2017 every bio scientist who wants to analyze dna knows that the process begins with the extraction of dna from cells of interest. This ph can be slowly lowered by extended water washing, or by using a buffer. Use andor alterations to the product, such as mixing with. Specifically designed to complement the inherent requirements of pcr, this pure, pipettable, smallscale resin is ready for downstream use. If the pdf does not display below, you may also download it here. This product is not regulated under the hazard communication standard, 29cfr1910. Vacuum or sweep up material and place in a disposal container section 7 handling and storage handling. Pdf extraction of acanthamoeba dna by use of chelex resin. Mediummesh resin is effective for both preparative and analytical protocols. Dispose of as nonhazardous waste in accordance with local, state and federal regulations. Safety data sheet according to 19072006 ec, article 31 iso 11014 printing date 18. I want to useproduct c7901, chelex 100, to make a metalfree solution. Chelex 100 sodium form 100200 mesh particle size sigmaaldrich.
These ingredients perform in concert to support your health when your body is challenged by environmental. Bt chelex 100 resin sodium form safety data sheet download 1432832, 1432836, 1432832edu, 1432834 msds ghsclp. Pdf a rapid and easy method for the dna extraction from. Sigmaaldrich material safety data sheet product name chelex.
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